Development of solid lipid nanoparticles loaded with retinoic acid and lauric acid for topical treatment of acne vulgaris
Elton Luiz Silva Department of Pharmaceutics, Faculty of Pharmacy
Federal University Minas Gerais
Brazil
Abstract:
Solid lipid nanoparticles (SLN) represent an alternative to overcome the side-effects associated with topical treatment of acne vulgaris with all-trans retinoic acid (RA). On the other hand, combination of retinoids and antimicrobials is an innovative approach in acne therapy. Recently, lauric acid (LA), a saturated fatty acid, has shown strong activity against Propionibacterium acnes. This work aims to develop SLN as carriers of RA and LA using stearylamine (SA) to increase the drug encapsulation efficiency (EE). SLN were prepared by the hot melt homogenization method using an emulsification-ultrasound. The EE was evaluated 0 and 15 days after preparation. The EE for RA in SLN with 0, 0,2 and 0,4% of SA were 100 ± 5, 100 ± 5 and 98 ± 7%, respectively, at initial time and 83 ± 3, 111 ± 1 and 100 ± 4%, 15 days later. The EE for LA were 106 ± 5, 97 ± 5 and 98 ± 1%, respectively, at initial time and 101 ± 1, 93 ± 1 and 44 ± 1%, 15 days later. Thus, SLN with 0,2% of SA showed higher EE for both acids and lower drug release after 15 days and was chosen for further biological studies.
